Abstract: Objective To comprehensively understand the map of transcripts during mitosis and their regulatory mechanisms of HAP1 cells by conducting transcriptome sequencing analysis after being released by mitotic synchronization arrest. Methods HAP1 cells were subjected to mitotic synchronous arrest with nocodazole and samples were collected after 0, 20, 80 min release, and RNA sequencing(RNA-seq) were performed. The transcriptome data was cleaned and the differentially expressed genes, expression trend clustering and functional enrichment combined with the protein interaction network were analyzed to explore the changes of signaling pathways in HAP1 cells during mitosis. Results The transcriptome of HAP1 cells after synchronous release from mitosis underwent significant changes in time series, and differential gene cluster analysis revealed four gene clusters were enriched in important biological processes such as p53 signaling and cytoplasmic translation. Conclusions The transcriptome time-dependent dynamic changes during mitosis in HAP1 cells are coordinated regulation of key signaling pathways including cellular stress response, translational control and chromatin remodeling, ensuring a balance between growth and stress response upon mitotic exit.

Key words: mitosis, transcriptome, cell cycle synchronization, signaling pathway regulation