Abstract: Objective To investigate the effect of lncRNA SNHG14 targeting at miR-142-5p on the neuronal damage induced by oligomeric Aβ. Methods PC12 cells were divided into control group, model group, model+si-NC group, model+si-SNHG14 group, model+miR-NC group, model+miR-142-5p group, model+si-SNHG14+anti-miR-NC group, and model+ si-SNHG14+anti-miR-142-5p group. The level of SNHG14 and miR-142-5p was calculated by RT-qPCR. CCK-8 method and flow cytometry were applied to detect inhibition rate on cell proliferation and apoptosis rate. ELISA method was used to detect TNF-αand IL-6 in the culture. Dual luciferase report assay was used to find the targeting relationship between SNHG14 and miR-142-5p. Results SNHG14 was highly expressed (P＜0.05)while miR-142-5p was lowly expressed (P＜0.05) in plasma of AD patients. Compared with the control group, the SNHG14 level, cell proliferation inhibition rate, and apoptosis rate in the model group were significantly increased (P＜0.05), the level of miR-142-5p was significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Compared with the model+si-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14 group were significantly reduced (P＜0.05), the level of TNF-α and IL-6 in the culture medium was significantly reduced (P＜0.05), IL-10 level was significantly increased(P＜0.05). Compared with the model+miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+miR-142-5p group were significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly reduced (P＜0.05) and IL-10 level was significantly increased (P＜0.05). miR-142-5p was a direct target of SNHG14. Compared with the model+si-SNHG14+ anti-miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+ si-SNHG14+anti-miR-142-5p group were significantly increased(P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Conclusions Silencing SNHG14 may alleviate oligomeric Aβ-induced neuronal damage by targeting at and promoting miR-142-5p expression.

Key words: Alzheimer's disease, SNHG14, miR-142-5p, oligomeric Aβ, nerve cell