Gelsemine inhibits proliferation and invasion of rheumatoid arthritis synovial fibroblast through down-regulating circ_001680

doi:10.16352/j.issn.1001-6325.2022.03.027

Abstract

Abstract: Objective To investigate the effect of gelsemine on the proliferation, migration and invasion of rheumatoid arthritis endothelial cells (RASFs) and its possible mechanism. Methods RASFs were isolated and cultured. After RASFs were intervened with 50, 100, 200 μg/mL gelsemine for 24 h or small interfering RNA or over-expression vector of circular RNA circ_001680 was transfected into RASFs, cell proliferation was measured by CCK-8 method and clone formation test, cell migration was detected by scratch test, cell invasion was examined by Transwell, the protein expression of E-cadherin and N-cadherin was detected by Western blot. The protein expression of circ_001680 was examined by RT-qPCR. Results Gelsemine or interference with circ_001680 expression reduced the A value, the number of clones, the healing rate of scratches and the number of invaded cells of RASFs and the protein expression of N-cadherin in cells (P＜0.05), and promoted the protein expression of E-cadherin (P＜0.05). Gelsemine reduced the expression of circ_001680 in RASFs (P＜0.05), and the over-expression of circ_001680 reduced the inhibitory effect of gelsemine on the proliferation, migration and invasion of RASFs. Conclusions Gelsemine may inhibit the proliferation, migration and invasion of RASFs, and its mechanism is potentially related to the down-regulation of circ_001680 expression.

Key words: gelsemine, rheumatoid arthritis, circ_001680, proliferation, migration, invasion

CLC Number:

R593.22

LIU Xin-bing, ZHANG Mei-hong. Gelsemine inhibits proliferation and invasion of rheumatoid arthritis synovial fibroblast through down-regulating circ_001680[J]. Basic & Clinical Medicine, 2022, 42(3): 429-435.

References

[1]Li J, Song Q, Shao L, et al. miR-124a inhibits proliferation and invasion of rheumatoid arthritis synovial fibroblasts[J]. Eur Rev Med Pharmacol Sci, 2018, 22:4581-4588.
[2]魏薇, 唐祎, 代婧, 等. 钩吻总碱通过JAK2/STAT3/Survivin通路调控人舌癌细胞株Tca8113增殖,凋亡的作用探讨[J]. 临床和实验医学杂志, 2020, 19:140-144.
[3]王文义, 檀兴慧, 张萍萍, 等. 钩吻总碱抑制人结肠癌细胞增殖及血管新生的作用研究[J]. 中国药房, 2020, 31:957-962.
[4]杨旋, 张梦洁, 张蓓. 高通量测序分析类风湿关节炎患者外周血单个核细胞circRNAs表达谱差异[J]. 免疫学杂志, 2019, 35:262-268.
[5]Zhao HD, Ma Y, Wu XS, et al. Circ_001680 aggravates the malignant process of gastric carcinoma by targeting MAP2[J]. Eur Rev Med Pharmacol Sci, 2020, 24:6072-6079.
[6]Jian XY, He H, Zhu JH, et al. Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340[J]. Mol Cancer, 2020, 19:20-35.
[7]李冠东, 颜则行, 孙水. 重组Furin蛋白对类风湿关节炎滑膜细胞生物学行为的影响[J]. 中国矫形外科杂志, 2017, 25:646-651.
[8]陈亮, 杨樱, 卢伊, 等. 钩吻总碱对人脐静脉内皮细胞增殖及迁移的影响[J]. 福建中医药大学学报, 2013, 23:41-43.
[9]邵鑫, 袁叶双, 蒋先虹, 等. 乌头碱对类风湿性关节炎成纤维细胞样滑膜细胞增殖,凋亡及自噬的影响[J]. 中国免疫学杂志, 2019, 35:3066-3070.
[10]王中扬, 吴祥, 包东桥, 等. 青藤碱对脂多糖诱导的人成纤维滑膜细胞增殖的影响[J]. 中药药理与临床, 2019, 35:46-50.
[11]李冠东, 颜则行, 孙水. 重组Furin蛋白对类风湿关节炎滑膜细胞生物学行为的影响[J]. 中国矫形外科杂志, 2017, 25:646-651.
[12]Mo JR, Zhao YW, Ao ZX, et al. Circ-APBB1IP as a prognostic biomarker promotes clear cell renal cell carcinoma progression through the ERK1/2 signaling pathway[J]. Int J Med Sci, 2020, 17:1177-1186.
[13]Gai SK, Sun L, Wang HY, et al. Circular RNA hsa_circ_0007121 regulates proliferation, migration, invasion, and epithelial-mesenchymal transition of trophoblast cells by miR-182-5p/PGF axis in preeclampsia[J]. Open Med (Wars), 2020, 15:1061-1071.
[14]Zhu YH, Zou CW, Jia YT, et al. Knockdown of circular RNA circMAT2B reduces oxygen-glucose deprivation-induced inflammatory injury in H9c2 cells through up-regulating miR-133[J]. Cell Cycle, 2020, 19:2622-2630.
[15]Zhong SP, Ouyang QQ, Zhu DJ, et al. Hsa_circ_0088036 promotes the proliferation and migration of fibroblast-like synoviocytes by sponging miR-140-3p and upregulating SIRT 1 expression in rheumatoid arthritis[J]. Mol Immunol, 2020, 125:131-139.