LncRNA expression profile in human malignant embryonic rhabdomyosarcoma cell line infected with Coxsackie virus group B type 5

doi:10.16352/j.issn.1001-6325.2022.01.011

Abstract

Abstract: Objective To explore the molecular mechanism of the interaction between Coxsackie virus group B type 5 (CVB5) and the host, The long non-coding RNA(lncRNA) expression profile in human malignant embryonic rhabdomyosarcoma cell line (RD) infected by CVB5 was investigated. Methods After 24 hours of infection with 1MOI CVB5 RNA was sampled and RNA-sequencing was used to identify the differential expression profile of lncRNA in the cells. Differentially expressed transcripts were analyzed by cluster analysis, GO analysis and KEGG pathway enrichment. At the same time, RNAfold was used to predict the secondary structure of lncRNA. Results Compared to the uninfected group, 1 754 mRNAs and 508 lncRNAs were up-regulated and 3 106 mRNAs/760 lncRNAs were down-regulated after CVB5 infection. Co-expressed genes that differentially express lncRNA were mainly enriched in biological processes such as molecular structure activity, protein molecular binding, and humoral immune response; lncRNA targets were mainly involved in olfactory transduction pathway, cytokine-cytokine receptors interaction, neuroactive ligand-receptor interaction and other pathways. In addition, the seven differen-tially expressed lncRNAs verified by quantitative real-time PCR (RT-qPCR) were consistent with the sequencing results. Conclusions lncRNA is mainly involved in the regulation of the immune processes, which lays the foundation for a full understanding of the regulatory role of lncRNA in CVB5 infection.

Key words: RNA sequencing, Coxsackie virus group B type 5, long non-coding RNA, RD cells, expression profile

CLC Number:

R373.2

TENG Pei-ying, LI Jing, SHI Yu-lu, YANG Fan, OU Xia, CHEN Wei. LncRNA expression profile in human malignant embryonic rhabdomyosarcoma cell line infected with Coxsackie virus group B type 5[J]. Basic & Clinical Medicine, 2022, 42(1): 68-74.

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