Protective effect of FK506 combined with hypothermic mechanical perfusion on donor liver of rats

Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (12): 1715-1718.

• Original Articles • Next Articles

Protective effect of FK506 combined with hypothermic mechanical perfusion on donor liver of rats

JIANG Li^1,2, LI Jian-hui², YU Hao³, LI Hao-yu², XIE Hai-yang², ZHOU Lin², ZHENG Shu-sen^2,3*

1. Laboratory Animal Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003;
2. NHC Key Laboratory of Combined Multi-organ Transplantation, Hangzhou 310003;
3. Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China

Received:2021-06-07 Revised:2021-10-12 Published:2021-12-03
Contact: *shusenzheng@zju.edu.cn

Abstract

Abstract: Objective To investigate the protective effects of FK506 combined with low temperature mechanical perfusion on the reduction of ischemia reperfusion injury of rat liver transplants. Methods SD rats were divided into HTK group and FK506 + HTK group. Orthotopic liver transplantation was performed 3 hours after machine perfusion of 150 mL HTK (HTK group) or FK506+150 mL HTK (FK506 + HTK group). Blood samples were collected at 3 and 24 hours after transplantation in both groups, and serum samples were centrifuged for liver function test. Twenty-four hours after transplantation, liver tissues were collected and fixed with formalin for HE staining. Results Serum ALT levels in FK506+HTK group were significantly lower than that in HTK group (P＜0.05). Inflammatory infiltration in FK506+HTK group was less than that in the HTK group, and the liver structure was intact. Conclusions FK506 added into perfusate for machine perfusion of the donor liver can effectively reduce liver function damage and inflammation damage within 3 hours after transplantation, and has a certain protective effect on the liver.

Key words: hypothermic machine perfusion, FK506, liver transplantation, ischemia reperfusion injury

CLC Number:

R617

JIANG Li, LI Jian-hui, YU Hao, LI Hao-yu, XIE Hai-yang, ZHOU Lin, ZHENG Shu-sen. Protective effect of FK506 combined with hypothermic mechanical perfusion on donor liver of rats[J]. Basic & Clinical Medicine, 2021, 41(12): 1715-1718.

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Protective effect of FK506 combined with hypothermic mechanical perfusion on donor liver of rats

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