Curcumin enhances the radio-sensitivity of prostate cancer cell line PC-3

Abstract

Abstract: Objective To explore the molecular mechanism of curcumin increasing the radiosensitivity of prostate cancer PC-3 cells. Methods Prostate cancer PC-3 cells were cultured and treated with curcumin, irradiation or combined therapeutic methods to observe the proliferation, apoptosis and change of cell cycle of PC-3 cells shown by MTT,RT-qPCR and FCM. Results Compared with control group, the cells from the radiation or curcumin group, the survival rate, the level of PCNA mRNA and Cdc2 mRNA were decreased (P＜0.05), and the ratio of S phase and G₂/M phase, caspase-3 and caspase-9 activity and the apoptosis rate were increased (P＜0.05); Compared with the cells in the radiation group, after treated with curcumin combined with radiotherapy, the survival rate, the level of PCNA mRNA and Cdc2 mRNA were decreased (P＜0.05), and the ratio of S phase and G₂/M phase, caspase-3 and caspase-9 activity and the apoptosis rate were all increased (P＜0.05). Conclusions Curcumin has a certain radiosensitizing effect in vitro, which potentially strengthen the inhibitory effect of radiotherapy on the growth of prostate cancer PC-3 cells.

Key words: prostate cancer, curcumin, radiotherapy, sensitivity, apoptosis

CLC Number:

R453

CHENG Ji, FU Guo, ZHAO Hua-cai, WANG Zhi-gang, GAO Dan, DONG Qing-chuan. Curcumin enhances the radio-sensitivity of prostate cancer cell line PC-3[J]. Basic & Clinical Medicine, 2021, 41(12): 1792-1796.

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