miR-26a inhibits the proliferation, migration and invasion of uterine leiomyoma cells through targeting IGF-1

Abstract

Abstract: Objective To investigate the effect and mechanism of miR-26a which targets at insulin like growth factor 1(IGF-1) on the proliferation, migration and invasion of uterine leiomyoma cells. Methods The primary cultured uterine leiomyoma cells were divided into miR-NC group(mimics negative control sequence and was transfected into cells), miR-26a group(miR-26a mimics was transfected into cells), (miR-26a+pcDNA3.1) group(miR-26a mimics and blank vector were transfected into cells) and (miR-26a + IGF-1) group(miR-26a mimics and IGF-1 over-expression vector were transfected into cells). The expression of miR-26a was detected by RT-qPCR; MTT method and Transwell chamber were used to detect cell proliferation, invasion and migration. Western blot was used to detect the expression of PCNA, E-cadherin and MMP-2. Dual luciferase reporter gene assay verified the targeting relationship between miR-26a and IGF-1. Results The miR-26a expression of uterine leiomyoma cells in miR-26a group was significantly increased (P＜0.05). Compared with miR-NC group, the cell proliferation, migration and invasion in miR-26a group decreased significantly, the expression of PCNA and MMP-2 decreased significantly, while the expression of E-cadherin increased significantly (P＜0.05). There was a targeting relationship between miR-26a and IGF-1. Over-expression of IGF-1 attenuated the inhibition of miR-26a on the proliferation, migration and invasion of uterine leiomyoma cells and the expression of PCNA and MMP-2, and promoting effect of E-cadherin expression. Conclusions Over-expression of miR-26a may inhibit proliferation, migration and invasion of uterine leiomyoma cells by targeting at IGF-1.

Key words: uterine leiomyoma, miR-26a, insulin like growth factor 1, migration and invasion

CLC Number:

R737.33

XIANG Xue-qin, CUI Quan-zhe, TONG Yu-na, ZHAN Wen-bin, LU Jing. miR-26a inhibits the proliferation, migration and invasion of uterine leiomyoma cells through targeting IGF-1[J]. Basic & Clinical Medicine, 2021, 41(11): 1612-1617.

References

[1]Chuang TD, Xie Y, Yan W, et al. Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma[J]. Fertil Steril, 2018, 109:919-929.
[2]韩永贵, 孙长冬. 子宫肌瘤组织中肌球蛋白轻链激酶和硫酸基转移酶2A1表达量与细胞侵袭、上皮间质转化的相关性[J]. 中国妇幼保健, 2018, 33: 3535-3538.
[3]Xiao HJ, Ji Q, Yang L, et al. In vivo and in vitro effects of microRNA-124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway: miR-124 targeting JAG1 in GC via Notch pathway[J]. J Cell Biochem, 2018, 119:2520-2534.
[4]Cao D, Di M, Liang J, et al. MicroRNA-183 in cancer progression[J]. J Cancer, 2020, 11:1315-1324.
[5]杨璐西, 翟东霞, 张丹英, 等. mir-26a对子宫肌瘤细胞周期和增殖的影响[J]. 现代生物医学进展, 2016,16:4224-4227.
[6]Su C, Wang W, Wang C. IGF-1-induced MMP-11 expression promotes the proliferation and invasion of gastric cancer cells through the JAK1/STAT3 signaling pathway:[J]. Oncol Lett, 2018, 15:7000-7006.
[7]纪巍, 辛晓燕, 陈必良. IGF-1及其受体在子宫平滑肌瘤中的表达以及与雌、孕激素关系[J]. 临床军医杂志, 2009,37:127-129.
[8]Shao Y, Li P, Zhu ST, et al. miR-26a and miR-144 inhibit proliferation and metastasis of esophageal squamous cell cancer by inhibiting cyclooxygenase-2[J]. Oncotarget, 2016, 7:15173-15186.
[9]Gong Y, Wu W, Zou X, et al. MiR-26a inhibits thyroid cancer cell proliferation by targeting ARPP19[J]. Am J Cancer Res, 2018, 8:1030-1039.
[10]Gkioka E, Msaouel P, Philippou A, et al. The role of insulin-like growth factor-1 signaling pathways in uterine leiomyoma[J]. In Vivo, 2015, 29:637-649.
[11]Baird DD, Travlos G, Wilson R, et al. Uterine leiomyomata in relation to insulin-like growth factor-I, insulin, and diabetes[J]. Epidemiology, 2009, 20:604-610.
[12]Tan X, Fan S, Wu W, et al. MicroRNA-26a inhibits osteosarcoma cell proliferation by targeting IGF-1[J]. Bone Res, 2015, 3:30-35.
[13]Long ZW, Wu JH, Hong C, et al. miR-374b promotes proliferation and inhibits apoptosis of human GIST cells by inhibiting PTEN through activation of the PI3K/Akt pathway[J]. Mol Cells, 2018, 41:532-544.
[14]Wang W, Yue Z, Tian Z, et al. Expression of Yin Yang 1 in cervical cancer and its correlation with E-cadherin expression and HPV16 E6[J]. PLoS One, 2018, 13:e0193340. doi: 10.1371/journal.pone.0193340.
[15]Bogusiewicz M, Stryjecka-Zimmer M, Postawski K, et al. Activity of matrix metalloproteinase-2 and -9 and contents of their tissue inhibitors in uterine leiomyoma and corresponding myometrium[J]. Gynecol Endocrinol, 2007, 23:541-546.