Erastin induces cell apoptosis in human non-small cell lung cancer cell line A549

Abstract

Abstract: Objective To explore whether erastin induces apoptosis and to investigate the molecular mechanism in A549 cells. Methods A549 cells were treated with erastin at different concentrations, with or without ROS scavenging agent N-acetyl-L-cysteine (NAC) and with or without apoptosis inhibitor (Z-VAD-FMK). Then iron content was measured with Iron Assay Kit, cell injure and apoptosis with CCK-8 and flow cytometry in A549 cells. Results Erastin induced the increase of iron content and followed by the injury of A549 cells. Erastin further induced apoptosis of A549 cells (P＜0.05), which was inhibited by apoptosis inhibitor Z-VAD-FMK. Erastin induced an increase in peroxides (ROS) then followed by apoptosis(P＜0.05), which was inhibited by the ROS scavenging agent NAC(P＜0.05). Conclusions Erastin has an inhibitory effect on NSCLC A549 by inducing intracellular ROS production, which provides evidences for the pathogenesis, diagnosis and treatment of NSCLC.

Key words: erastin, non-small cell lung cancer, apoptosis

CLC Number:

R394-33

JIANG Xue-dong, LI Wen-ya. Erastin induces cell apoptosis in human non-small cell lung cancer cell line A549[J]. Basic & Clinical Medicine, 2021, 41(9): 1313-1317.

References

[1]Smolle E, Leithner K, Olschewski H. Oncogene addiction and tumor mutational burden in non-small-cell lung cancer: Clinical significance and limitations[J]. Thorac Cancer, 2020, 11: 205-215.
[2]Battaglia AM, Chirillo R, Aversa I, et al. Ferroptosis and cancer: mitochondria meet the “Iron maiden” cell death[J], Cells, 2020, 9: 1505. doi: 10.3390/cells9061505.
[3]徐飞,陈维达,郭溟浩, 等. 铁死亡调控机制及在肺癌治疗中的研究进展[J]. 基础医学与临床, 2021, 41: 442-447.
[4]Wang M, Mao C, Ouyang L, et al. Long noncoding RNA LINC00336 inhibits ferroptosis in lung cancer by functioning as a competing endogenous RNA[J]. Cell Death Differ, 2019, 26: 2329-2343.
[5]Chen P, Wu Q, Feng J, et al. Erianin, a novel dibenzyl compound in dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis[J]. Signal Transduct Target Ther, 2020, 5: 51. doi: 10.1038/s41392-020-0149-3.
[6]Li Y, Yan H, Xu X, et al. Erastin/sorafenib induces cisplatin-resistant non-small cell lung cancer cell ferroptosis through inhibition of the Nrf2/xCT pathway[J]. Oncol Lett, 2020, 19: 323-333.
[7]Lin X, Ping J, Wen Y, et al. The mechanism of ferroptosis and applications in tumor treatment[J]. Front Pharmacol, 2020, 11: 1061. doi: 10.3389/fphar.2020.01061.
[8]Wang J, Deng B, Liu Q, et al. Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload[J]. Cell Death Dis, 2020, 11: 574. doi: 10.1038/s41419-020-02777-3.
[9]Li C, Dong X, Du W, et al. LKB1-AMPK axis negatively regulates ferroptosis by inhibiting fatty acid synthesis[J]. Signal Transduct Target Ther, 2020, 5: 187. doi: 10.1038/s41392-020-00297-2.
[10]Su LJ, Zhang JH, Gomez H, et al. Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis[J]. Oxid Med Cell Longev, 2019, 2019: 5080843. doi: 10.1155/2019/5080843.
[11]Espinosa-Oliva AM, García-Revilla J, Alonso-Bellido IM, et al. Brainiac caspases: beyond the wall of apoptosis[J]. Front Cell Neurosci, 2019, 13: 500. doi: 10.3389/fncel.2019.00500.
[12]Pan X, Lin Z, Jiang D, et al. Erastin decreases radioresistance of NSCLC cells partially by inducing GPX4-mediated ferroptosis[J]. Oncol Lett, 2019, 17: 3001-3008.
[13]Huang C, Yang M, Deng J, et al. Upregulation and activation of p53 by erastin-induced reactive oxygen species contribute to cytotoxic and cytostatic effects in A549 lung cancer cells[J]. Oncol Rep, 2018, 40: 2363-2370.

[1]	LUO Menglin, ZHENG Feng, JI Xinyao, QI Caihong, XIAO Yuheng, NIU Changchun. Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1 [J]. Basic & Clinical Medicine, 2024, 44(9): 1236-1242.
[2]	WANG Tingting, WEI Huan, YANG Yongli, ZHOU Xian, HU Yang. Nervous protection provided by notoginsenoside Rg1 in rat model with Alzheimer′s disease [J]. Basic & Clinical Medicine, 2024, 44(8): 1094-1100.
[3]	GAO Chao, ZHANG Runhan, WANG Wei, ZHAO Manting, JIAO Yan, LI Zhe. Effect of artesunate on neuroinflammation in depressed mouse model by regulating cGAS-STING signaling pathway [J]. Basic & Clinical Medicine, 2024, 44(8): 1126-1132.
[4]	YU Dongling, MO Shaoe, FU Wen, CHEN Shi, XIE Chouqin, LAN Yuyan. Inhibition of sigma-1 receptor reduces DRG cell apoptosis of rats with neuropathic pain [J]. Basic & Clinical Medicine, 2024, 44(8): 1101-1106.
[5]	ZHENG Hong, CHEN Xiaoxia, HAN Lizhou, CHEN Miao, HUANGFU Juan. Decrease of lncRNA-RMRP expression inhibits proliferation and invasion of human lung cancer cell line A549 [J]. Basic & Clinical Medicine, 2024, 44(7): 974-978.
[6]	GAO Yinjie, TONG Anli. Mechanisms of cell death and proliferation of aldosterone-producing adenoma [J]. Basic & Clinical Medicine, 2024, 44(6): 758-762.
[7]	LI Jinping, LIU Hanqing, YANG Quanhui. Change of cell cycle in rat spleen after severe abdominal infection [J]. Basic & Clinical Medicine, 2024, 44(6): 828-832.
[8]	GONG Jintao, LI Jianwei, LI Yuheng, LI Qian, ZHAO Chunhua. Bone marrow-derived mesenchymal stem cells relieve the adverse effects of simulated microgravity on mouse cerebral cortex [J]. Basic & Clinical Medicine, 2024, 44(6): 772-778.
[9]	LIANG Xiangcun, WEI Xiaoyu, LIANG Jian, WANG Qing, GENG Guang. Anlotinib inhibits proliferation and promotes apoptosis of human non-small cell lung cancer cell lines through miR-16-5p/PD-1 axis [J]. Basic & Clinical Medicine, 2024, 44(4): 503-512.
[10]	YANG Chunsheng, WANG Tianxing, ZHANG Tiecheng, WU Hengmin, WANG Baolan. Effects of biological clock gene Bmal1 on the expression of cell cycle-associated genes in chondrocytes [J]. Basic & Clinical Medicine, 2024, 44(4): 496-502.
[11]	YANG Kaifei, ZHU Jingge, ZHANG Yangyang, ZHAO Junguo, GAO Yuyue, HU Huanhuan, JI Guojie. Effect of sorafenib induced apoptosis and autophagy on drug resistance in HeLa cells [J]. Basic & Clinical Medicine, 2024, 44(4): 467-473.
[12]	LI Na, LI Tao, YANG Dongli, YAO Yuan. Honokiol inhibits the proliferation of human adipose-derived mesenchymal stem cells [J]. Basic & Clinical Medicine, 2024, 44(4): 483-488.
[13]	ZHANG Jing, YANG Zigeng, CAI Wenqin, CAO Weiwei, WEI Hongmei, XUE Xixi, WU Bin. Inhibition of prohibitin 2 enhances the sensitivity of non-small cell lung cancer cell line A549 to erlotinib [J]. Basic & Clinical Medicine, 2024, 44(3): 325-332.
[14]	AN Qi, QI Guangzhao, HAN Chao. Aucubin inhibits the proliferation of human hepatocellular carcinoma cells line HepG2 [J]. Basic & Clinical Medicine, 2024, 44(3): 333-338.
[15]	LI Juan, LI Xiaofeng, XU Shengzhi, TANG Kai. Efficacy of cinobufacini capsule combined with docetaxel plus cisplatin chemotherapy for the treatment of non-small cell lung cancer [J]. Basic & Clinical Medicine, 2024, 44(2): 247-251.