Influence of splicing factor MBNL3 on malignant biology behavior of pancreatic cancer cells

Abstract

Abstract: Objective To investigate MBNL3 expression and its effect on malignant biology behavior of pancreatic cancer cells. Methods Fifty paraffin samples of pancreas cancer excised during surgery in Peking Union Medical College Hospital from 2017 to 2020 were collected. Immunohistochemistry was conducted to explore expression of MBNL3. Pancreatic cancer cells were treated with MBNL3 knocked down or over-expressed by transfection. The proliferation and invasion or migration in different pancreas cells were examined respectively by real time cellular analysis and Transwell assay. The colony formation in different pancreas cells were examined by plate colony forming test. The MBNL3 expression in cell lines was analyzed by Western blot. Results Overall staining score of MBNL3 in pancreatic cancer tissues was 5.0±2.7, significantly higher than that of adjacent tissues(2.1±0.6)(P＜0.05). Cell proliferation experiment showed that 72 h after transfection, MBNL3 knockdown groups grew slower than control group in pancreatic cancer cells, whereas MBNL3 over-expressed groups grew faster than control group. Transwell assay showed that 10 h after transfection, invasion and migration of MBNL3 knockdown groups were weaker than control groups in pancreatic cancer cells (P＜0.05),meanwhile MBNL3 over-expressed groups were stronger than control groups in pancreaticcancer cells (P＜0.05). Colony forming experiments showed that colony formation number of MBNL3 knockdown groups were less than control groups in pancreatic cancer cells (P＜0.05), and the same of MBNL3 over-expressed groups were more than control group in pancreatic cancer cells(P＜0.05). Conclusions MBNL3 is highly expressed in pancreatic cancer tissue, which might take part in regulating the proliferation, invasion and migration of pancreatic cancer cells.

Key words: pancreatic cancer, splicing factor, invasion, migration

CLC Number:

R576

HAO Wen-zhen, CHEN Jie-min, FENG Yun-lu, WU Xi, WANG Qiang, WU Dong, YANG Ai-ming. Influence of splicing factor MBNL3 on malignant biology behavior of pancreatic cancer cells[J]. Basic & Clinical Medicine, 2021, 41(5): 688-693.

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