Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2

Abstract

Abstract: Objective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication dilated cardiomyopathy cardiomyocyte group (ADR group), APS intervention (APS group), transfection anti-miR-NC (APS+anti-miR-NC group) and transfected anti-miR-4701-3p(APS+anti-miR-4701-3p group). Flow cytometry was applied to determine apoptosis, Western blot was employed to test B cell lymphoma/leukemia-2(Bcl-2), Bcl-2 related X protein (Bax), nuclear related antigen Ki-67(Ki-67) and proliferation cell nuclear antigen (PCNA) protein expression, enzyme-linked immunoassay (ELISA) method was used to detect B-type brain natriuretic peptide (BNP) level. Cell proliferation was detected by commercially available kit 8(CCK-8), and real-time quantitative PCR (RT-qPCR) was used to analyze the microRNA-4701-3p(miR-4701-3p) expression. Results Adriamycin significantly increased BNP content, apoptosis rate, and Bax protein level in H9c2 cells, and greatly reduced Bcl-2 protein expression(P＜0.05). Angelica polysac-charide obviously increased H9c2 cell viability, Ki-67, PCNA, Bcl-2 protein expression and miR-4701-3p expression after doxorubicin induction, and remarkably reduced cell apoptosis and Bax protein expression(P＜0.05). Knockdown of miR-4701-3p significantly reduced the cell viability, Ki-67, PCNA, and Bcl-2 protein levels of dilated cardiomyopathy H9c2 cells treated with angelica polysaccharide, while significantly increased its apoptosis rate and Bax protein expression(P＜ 0.05). Conclusions Angelica polysaccharide up-regulates the expression of miR-4701-3p, promotes the proliferation of adriamycin-induced rat cardiomyocyte line H9c2.

Key words: adriamycin, cell line H9c2, miR-4701-3p, BNP

CLC Number:

R542.2

YANG Ping, DAI Tian, ZHANG Su-chuan. Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2[J]. Basic & Clinical Medicine, 2021, 41(4): 551-557.

References

[1] Athanasios B, Konstantinos R, Aris A. Current perspectives on the diagnosis and management of dilated cardiomyopathy, beyond heart failure: a cardiomyopathy clinic doctor's point of view[J]. Hellenic J Cardiol, 2018, 59: 254-261.
[2] Mitrut R, Stepan AE, Pirici D. Histopathological aspects of the myocardium in dilated cardiomyopathy.[J]. Curr Health Sci J, 2018, 44: 243-249.
[3] Nakamori S, Dohi K, Ishida M, et al. Native T1 mapping and extracellular volume mapping for the assessment of diffuse myocardial fibrosis in dilated cardiomyopathy[J]. JACC Cardiovasc Imaging, 2018, 11: 48-59.
[4] Du G, Chen J, Wang Y, et al. Differential expression of STAT-3 in subtypes of oral lichen planus: a preliminary study[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2018, 125: 236-243.
[5] Niu X, Zhang J, Ni J, et al. Network pharmacology-based identification of major component of Angelica sinensis and its action mechanism for the treatment of acute myocardial infarction[J]. Biosci Rep, 2018, 38.doi: 10.1042/BSR20180519.
[6] 霍礼超, 李梦丽, 乔成栋. 当归多糖对缺氧-复氧损害H9c2心肌细胞的保护作用[J]. 岭南心血管病杂志, 2019, 25: 579-585.
[7] 热斯拉特·艾力木. 维医异常黑胆质型消化系统肿瘤患者外周血共性miRNA标记物的研究[D]. 2015:22.
[8] 曾志鹏. microRNA-451a在扩张型心肌病患者免疫异常中的作用及机制研究[D]. 华中科技大学, 2017:2.
[9] 朱文晖, 朱芳, 张白雪, 等. LY294002对扩张型心肌病大鼠模型心肌结构及心功能的影响[J]. 中南大学学报(医学版), 2018, 43: 35-40.
[10] 郑博宇, 俸艳英, 阳志军. 缬沙坦对阿霉素所致心肌损伤保护作用的相关机制研究[J]. 广西医科大学学报, 2019, 36: 533-537.
[11] Xie D, Liao Y, Wu B, et al. Cardiac Nestin⁺ cells derived from early stage of dilated cardiomyopathy enhanced the survival of the doxorubicin-injured cardiac muscle HL-1 cells[J]. Int Heart J, 2018, 59: 180-189.
[12] 霍礼超, 李梦丽, 乔成栋. 当归多糖对缺氧-复氧损害导致的H9c2心肌细胞能量代谢障碍的保护作用[J]. 河北医学, 2019, 25: 568-572.
[13] Hui P, Linlin Z. Angelica sinensis, polysaccharide protects rat cardiomyocytes H9c2 from hypoxia-induced injury by down-regulation of microRNA-22[J]. Biomed Pharmacother, 2018, 106: 225-231.
[14] Wang HY, Ma YR, Lin YF, et al. SHU00238 promotes colorectal cancer cell apoptosis through miR-4701-3p and miR-4793-3p[J]. Front Genet, 2020, 10: 1320-1326.