Jagged-1 inhibits mouse embryonic stem cells differentiating into haematopoietic stem or progenitor cells

Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (1): 55-61.

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Jagged-1 inhibits mouse embryonic stem cells differentiating into haematopoietic stem or progenitor cells

CHEN Ri-lin¹, ZHENG Wei-rong², TAN Lin³, LIU Dong-qiang³, SHI Hui³, CHEN Qi-kang^1*

1. Department of Pediatrics,the Affiliated Shunde Women and Children Hospital, Guangdong Medical University, Shunde 528300;
2. Department of Pediatrics, the First Hospital of Quanzhou,Quanzhou 362000;
3. Children's Medical Center, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, China

Received:2019-05-16 Revised:2020-03-30 Online:2021-01-05 Published:2020-12-30
Contact: ^*chen.qikang@163.com

Abstract

Abstract: Objective To investigate the effect of Notch signaling pathways on differentiation of mouse embryonic stem cells(ESC) into haematopoietic stem or progenitor cells(HSC/HPC). Methods 1)incubate mouse embryoid body cells and divide the embryonic stem cells into five groups as EB group,control group,Jagged-1 group, DAPT group and Jagged-1-DAPT group. 2)detect the phenotype of HSC/HPC by flow cytometry. 3)The expression of Notch signaling pathway, mouse embryonic stem cell phenotype and HSC/HPC phenotype were detected by RT-PCR. Results 1)the number of embryonic stem cells in Jagged-1 group was significantly more than that in control group and Jagged-DAPT group(P＜0.05);2)the number of HSC/HPC in Jagged-1-DAPT group was significantly more than that in control group and in Jagged-1 group(P＜0.05); 3)the expression of Notch1, Notch2 and Notch4 mRNA in Jagged-1 group was significantly higher than that in control group(P＜0.05), 4)the expression of Notch1 and Notch4 mRNA in DAPT group and Jagged-1-DAPT group was lower than that in control group(P＜0.05). Conclusions Jagged-1 activation of Notch signaling pathway potentially inhibits ESC differentiation into HSC/HPC in mice model.

Key words: embryonic stem cells, Notch signal pathway, hematopoietic stem cells, Jagged-1

CLC Number:

R329.28
Q253

CHEN Ri-lin, ZHENG Wei-rong, TAN Lin, LIU Dong-qiang, SHI Hui, CHEN Qi-kang. Jagged-1 inhibits mouse embryonic stem cells differentiating into haematopoietic stem or progenitor cells[J]. Basic & Clinical Medicine, 2021, 41(1): 55-61.

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Jagged-1 inhibits mouse embryonic stem cells differentiating into haematopoietic stem or progenitor cells

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