CD151 promotes the proliferation and invasion of colorectal cancer cell line HT29

Abstract

Abstract: Objective To study the effect of CD151 on proliferation and invasion of colorectal cancer cell line HT29 and the correlated mechanism. Methods The cells were divided into HT29 cell group and CD151-HT29 cell group. The expression of CD151 was detected by RT-qPCR; the protein expression of CD151, LRG-1, LGR-5 and β-catenin was detected by Western blot. The proliferation and invasiveness of HT29 and CD151-HT29 cells were observed by MTS proliferation, cloning and Transwell chamber assay. Results The expression of CD151 in CD151-HT29 cells was significantly lower than that in HT29 cells (P＜ 0.05); the proliferation and invasion ability of CD151-HT29 cells were significantly less than HT29 cells (P＜ 0.05). The protein expression of LRG-1, LGR-5 and β-catenin in CD151-HT29 cells was significantly lower than that in HT29 cells. Conclusions CD151 can promote the proliferation and invasion of colorectal cancer.

Key words: CD151, colorectal cancer, β-catenin

CLC Number:

R735.3

LIU Xue-gang, LIU Yan-cai, WU Chun-ping, LI Jing-guang, ZHAO Ling-ling, ZHANG Hao, ZHANG Zhen-ya. CD151 promotes the proliferation and invasion of colorectal cancer cell line HT29[J]. Basic & Clinical Medicine, 2020, 40(12): 1626-1630.

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