Abstract: Objective To investigate the relevance of the expression of DPY30 and ASH2L, two core members of trithorax group proteins (TrxG). Methods Northern blot was used to detect the expression of ASH2L and DPY30 in various human tissues and the trend of ASH2L and DPY30 expression during the differentiation of human chronic myelogenous leukemia (K562) cells induced by PMA. Western blot and RT-qPCR were used to detect the expression of protein and RNA of TrxG core components in the cerebral cortex of ASH2L conditional knockout mouse; IGV software was used to visually analyze the distribution of ASH2L and H3K4me3 on ASH2L and DPY30 gene chromatin in ChIP-seq results of mouse cerebral cortex. Results The expression of DPY30 and ASH2L similar in various tissues of the human body; The expression of DPY30 and ASH2L was both gradually decreasedPMA-induced differ- entiation of K562 cells; DPY30 protein level decreased in the cerebral cortex of ASH2L-cKO mouse while the expression change of DNA level of DPY30 has no statistically significant. Conclusions DPY30 has a correlation with ASH2L expression and ASH2L may regulate the protein expression level of DPY30.

Key words: DPY30, ASH2L, gene expression