miR-1908 inhibits apoptosis of high glucose-induced human retinal vascular endothelial cell line HRECs

Abstract

Abstract: Objective To investigate the effect of miR-1908 on apoptosis of human retinal vascular endothelial cells (HRECs) induced by high glucose concentration and its molecular mechanism. Methods HRECs cells were cultured and divided into normal group and high glucose group. The expression of miR-1908 was detected by RT-qPCR. miR-1908 mimics or small interfering RNA of ILK was transfected into HRECs cells, the expression miR-1908 and ILK protein was detected by RT-qPCR and Western blot in order to detect the transfection efficiency, respectively. Then high concentration of glucose was used to interfere with HRECs with miR-1908 over-expressed or ILK expression inhibited. Apoptosis rate was detected by flow cytometry, Bcl-2 and Bax protein expression was detected by Western blot. The dual luciferase reporter gene assay validated the relationship between miR-1908 and ILK. Results Compared with the control group, the expression level of miR-1908 in HRECs of high glucose group was significantly decreased (P＜0.05). miR-1908 over-expressed of or ILK expression decreased the apoptosis rate of HRECs induced by high concentration of glucose (P＜0.05), up-regulated Bcl-2 protein expression (P＜0.05), and down-regulated Bax protein expression (P＜0.05). miR-1908 negatively regulated ILK expression, and over-expression of ILK partially reversed the effect of miR-1908 on apoptosis rate, Bcl-2 and Bax protein expression in HRECs. Conclusions miR-1908 up-regulates the expression of Bcl-2 protein and down-regulates the expression of Bax protein to inhibit the apoptosis of HRECs via negatively regulating ILK expression.

Key words: retinal vascular endothelial cells, high glucose level, miR-1908, integrin-linked kinase, cell apoptosis

CLC Number:

R774.5

ZHOU Li-ping, MAO Xiao-chun, LI Qin. miR-1908 inhibits apoptosis of high glucose-induced human retinal vascular endothelial cell line HRECs[J]. Basic & Clinical Medicine, 2020, 40(8): 1090-1095.

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