RNA localization in cytoplasm and nucleus of human embryonic stem cells

Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (6): 765-770.

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RNA localization in cytoplasm and nucleus of human embryonic stem cells

ZHOU Fan-qi¹, FENG Wen-xin², TAN Pu-wen³, MA Yan-ni¹, WANG Dong³, YU Jia^1*

1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. Henan Geology Vocational College, Zhengzhou 450000;
3. Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510000, China

Received:2020-03-08 Revised:2020-04-19 Online:2020-06-05 Published:2020-05-29
Contact: *j-yu@ibms.pumc.edu.cn

Abstract

Abstract: Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified.

Key words: human embryonic stem cells (hESCs), RNA localization

CLC Number:

Q522

ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia. RNA localization in cytoplasm and nucleus of human embryonic stem cells[J]. Basic & Clinical Medicine, 2020, 40(6): 765-770.

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RNA localization in cytoplasm and nucleus of human embryonic stem cells

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