Abstract: Objective To explore the molecular mechanism of anti-glioma by clofoctol targeting on RNA-binding proteins. Methods Biotin pull-down experiments were used to detect the binding of RNA-binding protein UNR (upstream of N-ras) to the target gene Krüppel-like factor 13(KLF13) mRNA fragments, and the effect of clofoctol on the binding to UNR to KLF13 mRNA fragments; The results of clofoctol DARTS sample's mass spectrometry combined with transcriptome chip analysis were used to screen other RNA-binding protein targets and to predict other downstream target genes. Results Clofoctol enhanced the interaction between UNR and the fragment of KLF13 mRNA; Clofoctol also acted on other RNA-binding proteins, and functioned in mTOR signaling pathway and TNF-related apoptosis-inducing ligand (TRAIL)-activated apoptotic signaling pathway. Conclusions Clofoctol can resist gliomas by acting on RNA-binding proteins.

Key words: clofoctol, glioma, UNR, KLF13 mRNA, RNA-binding proteins