Association between SHMT1 gene promoter methylation and ischemic stroke

Abstract

Abstract: Objective To explore the relationship between serine hydroxymethyltransferase (SHMT1) gene methylation and ischemic stroke patients. Methods The quantitative methylation-specific PCR was used to measure the level of SHMT1 promoter methylation in 290 healthy control individuals and 141 ischemic stroke patients. Results The results showed that the SHMT1 methylation level in the stroke group was 24.87(16.97~35.46), higher than that in the control group 6.58(2.43~15.14) (P＜0.05). After adjusting for relevant risk factors, SHMT1 methylation was a risk factor for stroke(OR=1.051, 95% CI= 1.034~1.068). Area under the curve was 0.804, 95% CI=0.760~0.849(P＜0.01). The level of uric acid was associated with SHMT1 methylation in the control group(r_s=0.17, P＜0.01), and the level of TG was associated with SHMT1 methylation in the stroke group(r_s=0.18, P＜0.05). SHMT1 methylation expression was negatively correlated with mRNA expression(r=-0.472, P＜0.01). Conclusions The SHMT1 gene is more methylated and results in low expression in ischemic stroke patients, so SHMT1 hypermethylation is a risk factor for stroke.

Key words: ischemic stroke, serine hydroxymethyltransferase 1, DNA promoter, methylation

CLC Number:

R394.3

HU Jing-cen, LI Lian, WANG Shu-yu, XU Lei, YANG De-lun, HAN Li-yuan, XU Guo-dong. Association between SHMT1 gene promoter methylation and ischemic stroke[J]. Basic & Clinical Medicine, 2020, 40(4): 451-455.

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