Abstract: Objective To investigate the effects of ribosomal protein 25 (RPS25) on development and progression of ccRCC. Methods Quantitative Real-time PCR (qPCR), Western blot analysis, and dual luciferase reporter gene assays were conducted to examine RPS25 expression and c-Myc promoter activity in renal cancer tissue and renal cancer cell lines. Proliferation, migration and invasion of renal cancer cells either exhibiting knockdown of RPS25 or its downstream gene c-Myc were assessed by MTS and Transwell assay. Kaplan-Meier Plotter was used to analyze the survival of ccRCC patients with different RPS25 levels using data from The Cancer Genome Atlas consortia (TCGA). Results qPCR results showed that RPS25 mRNA was significantly increased in renal cancer cell lines and ccRCC samples. Mechanistic studies demonstrated that RPS25 promoted c-Myc expression by both translational control and transcription mechanisms. Particularly, coexpression of c-Myc reversed the depletion of RPS25-mediated tumor suppression. Kaplan-Meier Plotter showed that the increased RPS25 level correlated with poor survival. Conclusion This study demonstrates that RPS25 as an oncogene hae therapeutic potential and clinical values as a prognostic factor in ccRCC.

Key words: RPS25，c-Myc，clear cell renal cell carcinoma