Abstract: Objective To investigate the effect of high expression of PRDX6 on migration and invasiveness of lung cancer A549 cells. Methods 1) In vitro cultured human lung cancer A549 cells，divided into blank group for not transfection，experimental group for transfection PRDX6 and control group for empty plasmid transfection；2) The expression of PRDX6 in each group was detected by western blotting；3) The migration and invasion of cells in each group were assessed by transwell chamber assays. Results 1) The expression of PRDX6 protein in the experimental group was significantly higher than those in the blank and the control group(p﹤0.05).2) the capacity of A549 cells migration and Invasion in the experimental group was the highest among thethree groups(p﹤0.05).Conclusions The high expression of PRDX6 can enhance the migration and invasion of A549 cells.

Key words: A549 cells, PRDX6, Transfection, Invasion, migration