Abstract: Objective To explore the effects and mechanism of A2aR inhibitors(A2aRi) on proliferation, migration, apoptosis in A549 cells. Methods Experiments were divided into control group and intervention group, but the intervention group were treated by different concentrations of A2aR inhibitors. Cells proliferation determined by MTT method. A549 cells migration ability was detected by scratch experiment. Observing expression of A2aR and caspase3 determined by immunofluorescence staining. A2aR, caspase3 protein and A2aR mRNA expression determined by Western blot and RT-PCR . Results A2aRi could inhibit the proliferation of A549 cells（P<0.01）and migration ability of A549 cells were weaken. Red fluorescence intensity of A2aR is less than control; Green fluorescence intensity of caspase3 was enhanced. They expressed in cytoplasm. The expression of caspase3 protein was increased（P<0.01）, but the expression of A2aR protein was decreased（P<0.01）; and the expression of A2aR mRNA level was down-regulated. Conclusions A2aR is high expression in human non-small cell lung cancer cells, A2aRi can inhibit proliferation and migration ability of A549 cells.

Key words: non-smallcellLungcancer, Adenosine, Adenosinereceptor, apoptosis, Caspase3