Abstract: Object To explore the effects of rL-IL29 (recombinant Newcastle disease virus expressing IL-29 ) on endoplasmic reticulum stress, autophagy and apoptosis related protein in smll cell lung cancer cell line H446. Methods H446 cells were divided into control group, Newcastle Disease Virus NDV group and rL-IL29 group randomly, which the later groups infected with NDV and rL-IL29, respectively. Cell viability was detected by CCK-8 assay. IL-29 content in cell supernatant was detected by ELISA assay. Western blot and immunofluorescence were used to detect the expression of GRP78 and CHOP, which related with endoplasmic reticulum stress. Then, the experimental groups were treated with 4-PBA 4h, the expression of caspase-3 and LC3 I/II protein was detected with Western blot. Results The activity of cells decreased significantly with the increase of virus concentration, which increased significantly at 10-5 concentration (P<0.05). And the content of IL-29 in the supernatant of rL-IL29 group increased obviously than that of control group(P<0.05). The expression of GRP78, CHOP, LC3 I/II and caspase-3 had high level in NDV and rL-IL29 group than PBS group after infected 24 h. Moreover, rL-IL29 group had high level than NDV group (P<0.05). After pre-treated with 4-PBA 4h，the expression of GRP78, CHOP, LC3 I/II and cleaved-caspase-3 decreased in NDV and rL-IL29 group than control group (P<0.05). Conclusion rL-IL29 can promote the expression of endoplasmic reticulum stress, autophagy and apoptosis related protein in small lung cancer cells.

Key words: rL-IL29, lung cancer, endoplasmic reticulum stress, autophagy, apoptosis