Abstract: Objective To investigate effects of over-expression/knockdown SFRP5 on glucose metabolism in hepa1-6 hepatocarcinoma cells of mice. Methods Hepa1-6 hepatocarcinoma cell lines, established using adenovinus infection tool, are divided into three groups: control group(Ad-GFP), over-expression SFRP5 group(Ad-SFRP5), and knockdown SFRP5 group(Ad-shSFRP5). Glucose uptake rate was detected by glucose oxidase method(GOD). Gene expressions at mRNA and protein levels were determined by qRT-PCR and Western blotting. Results Compared with control group, over-expression SFRP5 increased glucose uptake rate, decresed PEPCK (P<0.05) and G6Pase expression (P<0.01)and increased insulin-stimulated InsR (P<0.01), AKT (P<0.05) phosphorylation in hepa1-6 cells. Knockdown SFFRP5 group showed an opposite result. Conclusions Over-expression SFRP5 could improve glucose metabolism through decreasing hepatic gluconeogenesis and enhancing insulin signaling pathway.

Key words: SFRP5, Hepa1-6, Glucose metabolism, Insulin resistance