Abstract: Objective To investigate whether adiponectin (APN) inhibits cardiomyocyte hypertrophy induced by high concentration of insulin by up-regulating AdipoR1 / AMPK / PI3K / AKT. Methods The cardiomyocytes of neonatal SD rats were isolated and cultured continuously for 9 days. Cardiomyocytes were divided into 4 groups according to different intervention conditions: control group (medium), model group (1×10-6 mol/L insulin), intervention group (1×10-6 mol/L insulin+7.5 mg/L adiponectin) and transfection group (AdipoR1 siRNA insulin + adiponectin processing after transfection). After 48 h, the average protein content, 3-methylhistidine (3-MH) content and myocardial cell surface area of each group were detected. Western blot was used to detect the expression of AdipoR1, AMPK, PI3K and AKT proteins. Results Compared with the control group, the average protein content of myocardial cells increased (P<0.05, n=3), the content of 3-MH decreased (P<0.05, n=10). Compared with the model group, the above changes in the intervention group were significantly reduced, while the expression of AdipoR1, AMPK, PI3K and AKT in the intervention group was increased (P<0.05, n=3) . Conclusion Adiponectin may inhibit rat cardiomyocyte hypertrophy induced by high concentration of insulin by up-regulating AdipoR1 / AMPK / PI3K / AKT.

Key words: heart failure, Myocyte hypertrophy, Adiponectin, AMPK, PI3K/AKT