Abstract: Objective To discuss the effect of breast cancer anti-estrogen resistance 1(BCAR1) knockdown on expression of p38 and p-p38 in lung cancer A549 cells. Methods Normal A549 cells (control group), A549 cells with RNAi letiviral vector of BCAR1 (interference group) and A549 cells with negative control letiviral vector (negative group) were cultured. Western blot was used to detect the expression of p38 and p-p38. The proliferation, cell cycle, migration and invasion were measured by colony formation assay, flow cytometer, Transwell experiment and scratch adhesion test, respectively. Results p-p38 expression in interference group cells was significantly lower than that in other two group cells（P<0.05）.G1 phase of interference group cells was significantly increasing than that in other two group cells（P<0.05）.The proliferation, migration and invasion of interference group cells were all significantly suppressed compared with that of other two group cells（P<0.05）. Conclusions BCAR1 knockdown decreases p-p38 expression and inhibits proliferation, migration and invasion of A549 cells.

Key words: BCAR1, P-P38, P38, A549 cells, lung cancer