Abstract: Objective To prepare polyclonal antibodies against mouse UPF1 protein and to investigate the expression of UPF1 protein during adipocyte differentiation. Methods UPF1 protein expression vector was constructed to prepare and purify rabbit UPF1 antibody. The differentation of 3T3-L1 cells were induced and to the expression of UPF1 was detected by CoIP. Results 1) High specific mUPF1 polyclonal antibody was prepared and the titer of this antibody reached 640000; 2) The expression of UPF1 protein does not change during adipogenesis; 3) In the process of adipocyte differentiation , interaction of UPF1 and UPF2 is increased. Conclusions 1) The polyclonal antibodies prepared by using 550 amino acids at the C terminal of mUPF1 protein could effectively recognize intact mUPF1 protein; 2)The interaction of UPF1 protein with UPF2 protein during adipocyte differentiation was enhanced.

Key words: Adipogenesis, UPF1, NMD, Antibody, Obesity