Abstract: Objective To investigate the effects of mangiferin on myocardial apoptosis induced by hypoxia ischemia in rats. Methods myocardial apoptosis model induced by hypoxia ischemia12h in rats. After mangiferin intervention, myocardial cell apoptosis was deteced by flow cytometry; apoptosis protease Caspase-3, Caspase-9 activity in cell lysates was detected by spectrophotometry; the protein expression of Bax and Bcl-2 was detected by western blot. Results myocardial apoptosis model was successfully established by hypoxia ischemia 12h. Compared with the model group, After mangiferin intervention, myocardial apoptosis rate was significantly decreased (P<0.05); apoptosis protease Caspase-3 and Caspase-9 activity were significantly decreased (P<0.05); the protein expression of the pro apoptotic protein Bax was significantly decreased (P<0.05), and the protein expression of the anti apoptotic protein Bcl-2 was significantly increased (P<0.05). Conclusion Mangiferin can significantly inhibit myocardial apoptosis induced by hypoxic-ischemic.

Key words: Mangiferin, hypoxia ischemia, myocardial apoptosis