Abstract: Objective To investigate the expression of Eotaxin-1 in clear cell renal cell carcinoma(ccRCC) and its relationship with tumor proliferation. Methods The Eotaxin-1 and PCNA expression from 58 tissues of ccRCC and para carcinoma were analysised by immunohistochemistry or western blot. Results Eotaxin-1 were mainly expressed in the cytoplasm while PCNA were expressed in the cell nucleus, positive rate of Eotaxin-1 and PCNA in ccRCC tissues was significantly higher than that in para carcinoma tissues(P＜0.05), positive rate of Eotaxin-1 and PCNA in pathologic stageⅡ/Ⅲ was significantly higher than that in pathologic stageⅠ(P＜0.01). The positive rate of Eotaxin-1 and PCNA in clinical stage Ⅲ/Ⅳ was significantly higher than that in clinical stageⅠ/Ⅱ(P＜0.05). The protein level of Eotaxin-1 and PCNA in ccRCC were significantly higher than that in para-carcinoma tissues(P＜0.01，P＜0.05), Eotaxin-1 was positively related to PCNA in ccRCC tissues(P＜0.05). Conclusion Eotaxin-1 was related to proliferation in ccRCC and was expected to be a clinical indicator for ccRCC.

Key words: ccRCC, Eotaxin-1, Western blotting