Abstract: Objective: To investigate the effect of SDF-1 / CXCR4 and SDF-1/ CXCR7 on the migration and proliferation of human umbilical cord mesenchymal stem cell. Methods: Using Ad-EASY adenoviral vector systems to construct recombinant adenovirus with overexpression of CXCR4 and CXCR7,hUC-MSCs was infected with Ad-CXCR4 or Ad-CXCR7 respectively, FCM was used to detect the expression of CXCR4 and CXCR7 as membrane receptors, Transwell assay was carried out to evaluate the SDF-1 induced migration ability of hUC-MSCs with CXCR4 or CXCR7 overexpression.MTT assay was performed to detect the cell proliferation activity for overexpression CXCR4 or CXCR7 of hUC-MSCs, as well as the cell survival after treated with H2O2. Results:The constructed adenovirus CXCR4 and CXCR7 successfully infected UC-MSCs, and the positive cell rate with membrane expression of CXCR4 or CXCR7 were 93.7% and 78.5%, respectively. Overexpression of CXCR4 and CXCR7 significantly enhanced SDF-1 induced cell migration of hUC-MSCs,which CXCR7 showed weaker effect, then CXCR7 but not CXCR4 was responsible for the SDF-1 induced cell viability and proliferation of hUC-MSCs. Conclusion: Both CXCR4 and CXCR7 mediated the SDF-1 induced migration of hUC-MSCs, in addition CXCR7 mediated the SDF-1 induced hUC-MSCs proliferation and cell viability under anti-oxidative stress.

Key words: CXCR4/CXCR7, Human umbilical cord mesenchymal stem cell, Cell migration, Cell proliferation