Abstract: Objective To determine the effect of platelet-derived growth factor-C (PDGF-C) on ovarian cancer cells（SKOV3 and A2780）cultured in vitro. Methods The ovarian cancer cells（SKOV3 and A2780）were cultured in vitro. Cryopreserved SKOV3 and A2780 was cultured after water bath at 37 ℃. CCK8 assay was used to detect the effect of PDGF -C (0, 10, 20, 30 and 40 μg/L) on cell proliferation. Flow cytometry was used to detect cell phases. Transwell assay and cell scratch test were used to detect cell migration. Results PDGF-C significantly induced the proliferation of SKOV3 and A2780, and increased the percentage of the cells at S phase. Its effect on the SKOV3 and A2780 proliferation was found in a dose-dependent manner. PDGF-C of 20 μg/L promoted the SKOV3 proliferation at a summit, and PDGF-C of 20 μg/L promoted the A2780 proliferation at a summit. PDGF-C also promoted the migration ability of cultured SKOV3 and A2780. Conclusions PDGF-C promotes the proliferation and migration of cultured VECs and MSCs.

Key words: platelet-derived growth factor-C, A2780, SKOV3, cell proliferation, migration