Abstract: Objective To investigate the effect of BPA on hepatocyte apoptosis in CD1 mice. Methods Forty-eight 4-week old male CD1 mice were randomly divided into 4 groups. Mice from different groups were exposed to different doses of BPA (0, 5, 50, 500μg/(kg?d)) through food contamination for 8 weeks. After the decapitation of mice and the isolation of liver, HE staining and Hoechst 33258 staining were used to observe the hepatocyte apoptosis; Western blot was performed to determine the protein expression of cleaved caspase3, Bax and Bcl-2 in liver; caspase-3 activity was detected using a caspase-Glo3/7 assay kit. Results Compared with the control group, mice treated with different concentrations of BPA showed enhanced apoptotic cells and caspase-3 activity in liver（P<0.01）, with significantly increased protein expression of cleaved caspase 3 and Bax（P<0.05）. Conclusions BPA induces hepatocyte apoptosis in CD1 mice.

Key words: Bisphenol A（BPA）, Apoptosis , Hepatocyte