Abstract: Objective To investigate of the expression changes of miR-363-3p in gastric cancer and its functional significance in HGC-27 cell line. Methods Real-time PCR analysis was used to detect the relative expression of miR-363-3p in 59 pairs of gastric cancer tissues and their normal control tissues. miR-363-3p mimics was transfected into HGC-27 cell line for miR-363-3p overexpression. The effects of miR-363-3p overexpression on cell proliferation, migration and invasion were detected by CCK-8, wounding heal and Transwell assays. Results Overexpression of miR-363-3p in gastric cancer cell line （HGC-27）. Proliferation text results showed that miR-363-3p could inhibit cell proliferation. Scratch text results showed that miR-363-3p could inhibit cell migration. Transwell text results showed that miR-363-3p could inhibit cell invasion. Conclusions miR-363-3p functions as a tumor suppressor miRNA in gastric cancer. Therefore it might have the potential to be a new target for gastric cancer therapy.

Key words: gastric cancer, miR-363-3p, proliferation, invasion, motility