Abstract: Objective To explore the effect of Clomiphene Citrate on endometrial proliferation and the mechanism. Methods Animals were divided into CC group， five rats in each Drug were given for five days，while other groups included CC+E2、CC、CO groups，5 rats each and drugs were given for ten days. Cycle was observed , and intragastrical administration in proestrus was carried out for ten days with: clomiphene citrate (CC), Estradiol (E2), Normal Saline (Control，CO). Proteins of uterus were extracted for Western blot. Results In drug treated group uterine growth factor decreased significantly compared with the control group. After E2 co-administration with CC, its growth signal factor was increased than that of CC group, but not restored to normal level. Conclusion CC is attributed to the inhibitor of endometrial hyperplasia by inhibiting uterine growth factor. Combinding E2 and CC can alleviate such symptoms. The possible molecular mechanisms is associated with PI3K/AKT、JNK、NFKB and other related pathways.

Key words: Comiphene Citrat, Estradiol, Endometrial Proliferation