Abstract: Objective To explore of the effect of miR-124-3p on the level of Tau phosphorylation through regulating caveolin-1 in Alzheimer，s diseases cell model. Mehods Wild type N2a (N2a/WT) and N2a/APPswe cells were cultured in vitro, real-time fluorescence quantitative PCR (Realtime-PCR) and Western blot（WB）was respectively used to detect the expression levels of miR-124-3p and amyloid precursor protein and caveolin-1; N2a/APPswe cell was respectively transfected by miR-124-3p mimics、caveolin-1 overexpression vector and caveolin-1 siRNA.Realtime-PCR and WB was respectively used to detect the expression levels of caveolin-1 and Tau and Tau-Ser404. Results Compared with Wild type N2a cell, in N2a/APPswe cell the expression of miR-124-3p was decreased (P＜0.01), while APP and caveolin-1 was increased (P<0.01). After N2a/APPswe cell was transfected with miR-124-3p mimics, the expression levels of caveolin-1 was decreased (P<0.01) and Tau-Ser404/Tau was decreased (P<0.01);after transfection of caveolin-1 overexpression vector, the expression levels of caveolin-1 was increased (P<0.01) and Tau-Ser404/Tau was increased (P<0.01); after transfection of caveolin-1 siRNA the expression levels of caveolin-1 was decreased (P<0.01) and Tau-Ser404/Tau was decreased (P<0.01). Conclusions miR-124-3p may reduce the level of Tau phosphorylation and play a neuroprotective role in AD through regulating the expression of caveolin-1.

Key words: miR124-3p, caveolin-1, N2a / APPswe cells, Tau protein, phosphorylation