Abstract: Objective To investigate the effects of cyclooxygenase-2 inhibitor celecoxib on proliferation, invasion and migration of human pancreatic cancer cell line PANC-1 and then determine the optimal concentration of celecoxib and the most suitable application time. Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of celecoxib (20、60、100μmol/L) for different durations (24、48、72h). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, Transwell invasion assay, and scratch assay respectively. Results The proliferation capability of PANC-1 cell was reduced by celecoxib in a concentration- and time-dependent manner (P<0.05). In addition, the invasion and migration capabilities were decreased by celecoxib in a concentration-dependent manner (P<0.01). Conclusions Celecoxib attenuates the proliferation of human pancreatic cancer cell line PANC-1 in a concentration- and time-dependent manner. Celecoxib attenuates the invasion and migration in a concentration-dependent manner.

Key words: Pancreatic cancer, celecoxib, proliferation, invasion, migration