Abstract: Objective To detect the repair capacity in rabbit cartilage defects by BMSCs cell sheets transfected by CDMP. Methods Rabbit bone marrow mesenchymal stem cells were Isolated and cultured in vitro; the passage 4 cells were tansfected with exogenous recombinant human cartilage-derived morphogenetic protein 1 by adenovirus vector; the transgenosis cell sheets were harvested by means of temperature-responsive culture dish after 7-14 days. After detecting the express of CDMP1 in transgenic cell sheet using Real Time PCR and Western blot, then the sheets were implanted into the rabbit thyroid cartilage defects .After 4 or 8 weeks, the culturing systems were analyzed at the gross level as well as at the histological level to observe the repair ability．The experiment was divide into three groups: （A）transfected by Ad-CMV-hCDMP1-IRES-eGFP BMSCs cell sheets；（B）transfected by Ad-CMV -eGFP BMSCs cell sheets；（C）BMSCs cell sheets . Results The express of CDMP1 in transgenosis cell sheets can be detected by Real Time PCR and Western blot; The detection of immunohistochemistry and Alcian Blue staining showed the expression of collagen II and GAG in group A，while negative expression in group B and group C. There was statistical difference between group A, group B and C (P<0.05). Conclusion CDMP transgenosis cell sheets had superior chondrogenic capacity ,which can effectively repair rabbit thyroid cartilage defects.

Key words: Cell sheet, CDMP1, BMSCs, Adenovirus infection, Cartilage tissue engineering