Abstract: Objective In this work, it showed that modifications are closely-associated with tissue-specific expression, function and development by generating the genome-wide maps of H3K9me3 of human heart and spleen. Methods The DNA of samples were extracted by Chromatin immunoprecipitation. The ChIP results were validated by qPCR.Then building the ChIP Sequencing library, contrasting with the target genome sequence to obtain compared Reads. And peak analysis the whole genome, GO enrichment analysis the biological functions of the related genes of peak. Results It found that 169 genes displayed significant H3K9me3 differences between heart and spleen. Among these genes, 64 genes are the special genes in heart-H3K9me3; 87 genes are the special genes in spleen-H3K9me3. From these genes, select eight genes of H3K9 which were significantly expression. Then selecting two genes of H3K9 which were the most significant difference expression from the eight genes.They are PTPN3 and RBMS. Conclusion Above the findings show the significance of H3K9me3 as a potential biomarker or promising target for epigenetic-based disease treatment.

Key words: H3K9me3, ChIP-seq, epigenetic