Abstract: Objective To study the effect of CCN5 on proliferation, migration and invasion of hepatoma carcinoma cells and the mechanism. Methods Hepatocyte High Expression System of CCN5 was constructed and transfected HepG2 cells to make CCN5 overexpression, and then cell proliferation detection reagent box and Transwell chamber were used to determine the effect of CCN5 overexpression on proliferation, migration and invasion of HepG2. To further study the mechanism, phosphorylation levels of Smad2 and p44/42MAPK were determined. Results CCN5 was overexpressed in HepG2 cells. After that, the proliferation, migration and invasion of the CCN5 overexpression group were significantly inhibited (P <0.05) compared with the normal control group. Phosphorylation levels of Smad2 and p44/42MAPK were also significantly inhibited. Conclusion Overexpression of CCN5 can inhibit proliferation, migration and invasion of HepG2, which plays an important role in the formation of human hepatoma and proposes a new idea for the prevention and treatment of hepatic carcinoma.

Key words: CCN5, Hepatoma carcinoma cell, Proliferation, Migration, Invasion