Abstract: Objective Comparison of polyethyleneimine package magnetic nanoparticles and lipofectamineTM 2000 in transfection of human adipose mesenchymal stem cells.And the feasibility, instantaneous interference efficiency and cell proliferation state, the transfection efficiency were compared. Methods The PEI-SPIO and lipofectamineTM 2000 connect REST-siRNA in vitro and transfect into human adipose mesenchymal stem cells, MTT method for determin the effects of human adipose mesenchymal stem cell proliferation and function. transfection efficiency was detected by fluorescence microscope.And REST changes were determined with Real-time PCR and Western-Blot. Results The survival rate of the PEI-SPIO method was 99% and the lipofectamineTM 2000 method was 79%(P<0.05); the transfection efficiency of PEI-SPIO was no difference, 75%和71% respectively, PEI-SPIO and lipofectamineTM 2000 as gene carrier at RNA level was (0.46±0.04) and (0.53±0.05) respectively,and at protein level was (0.501±0.006) and (0.523±0.004) respectively. Conclusion Magnetic nanoparticles because of its low toxicity can replace the lipofectamineTM 2000 transfect stem cells as a gene vector.

Key words: Key words: magnetic nanoparticles, polyethyleneimine, lipofectamineTM 2000 , human adipose mesenchymal stem cells, transfection