Abstract: Objective: Explore the role of fusion protein Pep-1-vMIP-II influence the expression of mice ARGs gene and in resistance to HIV-I infection. Methods: Smear the mouse back with protein Pep-1-vMIP-II, 2 hours later, and using immunohistochemical methods to evaluate the determination of vMIP-II in different tissues and organs penetrate. After smear the mouse back with protein for 24 hours, using real-time quantitative PCR to detect the ARGs mRNA expression levels in different tissues and organs.Graphpad Prism 5 software was used to analyze experimental data. Resμlts: Pep-1-vMIP-II can penetrate the cell membrane into the nucleus 2 hours later, and the distribution significantly increased in the kidney(P<0.05); After the smear of Pep-1-vMIP-II 24 hours, the expression levels of CCL5 mRNA were significantly increased in leucocytes, kidney, liver ,heart , lung (P<0.05),but there is no significant increase of CCL5 mRNA expression levels in the brain tissue; MX1, MX2 are upregμlated in a variety of tissues(P<0.05). Conclusions: Fusion protein Pep-1-vMIP-II can penetrate the skin into the kidney tissue in mice. Mainly through the activation of the CCL5 gene play an important regulatory role.

Key words: CPPs , Pep-1-vMIP-II, HHV-8