Abstract: Objective To investigate the effect of aquaporin 1 (AQP1) on human amniotic mesenchymal stem cells (hAMSCs) migration. Methods By immunofluorescence staining, RT-qPCR and Western blot, expression of AQP1 was detected on hAMSCs and migration ability of hAMSCs after siRNA interference was assessed with Transwell. Results The expression of AQP1 on hAMSCs was identified on membrance. After 48 hours interference, the expression of AQP1 was reduced by 60.13% for mRNA level and 40.42%for protein level. The cell numbers through the menberane of control group vs AQP1 siRNA group was signature reduction from 33.15±1.15 to 23.7±1.45, P<0.01. Conclusion AQP1 promoted migration of hAMSCs and involved in wound repair process.

Key words: AQP1, cell migration, human amniotic mesenchymal stem cells, wound repair