Abstract: Objective To study the systemic immune response intranasally immunized by type 71 whole virus inactivation antigen (EV71 Ag) combined with alpha mannan peptide (PA) adjuvant.. Methods Forty BALB/c mice that were five to six week old were randomly divided into five groups,10 mice per group. Immune groups were accepted EV71 Ag with or without adjuvants. Blood serum and bronchoalveolar lavages were collected at 0,2,3,4,5and6weeks since the first immune treatment.The level of IgG,IgA and neutralizing antibody in serum were detected by enzyme-linked immunosorbent assay(ELISA)and Neutralizing antibody tests repectively.MTT assay was involved to assess the proliferation of lymphocyte. Results The specific IgG antibody in serum could be detected in every group.There was no statistically significant different between the high-dose adjuvanted group and EV71Ag intramuscular injecting group（2.477±0.500 verse 3.040±0.120).EV71 Ag combined with alpha mannan peptide(PA)adjuvant caused lymphocyte proliferation in vitro,neutralizing antibody titer increased in all group.There were no significant difference between high-dose adjuvanted group and the EV71 Ag intramuscular injuecting group.Conclusion EV71 Ag combined with alpha mannan peptide(PA) adjuvant intranasally induced an effective systemic immune reponse.

Key words: Key words：Mucosal immunity, Enterovirus 71 antigen, Neutralizing antibody, specific IgG antibody, sIgA antibody, lymphocyte proliferation