Abstract: Objective To investigate the genetic stability of human bone marrow mesenchymal stem cells (HMSC-bm) under pulmonary adenocarcinoma microenvironment. Methods A co-cultured system of HMSC-bm and A549 cells was established through the combination of 6 well culture plate and transwell chamber as experimental group while HMSC-bm and A549 cells were cultured separately as the control groups. The morphology of the cells was observed by phase-contrast microscopy. The proliferation curve of mesenchymal stem cells was tested by MTT. Chromosome was examined by karyotyping analysis. The expression of HDAC4 protein was detected by Western blot. Results Cells in the co-culture group showed that the cell nucleus became bigger than the nucleus in HMSC-bm group and exhibited anachromasis in 7 days. The proliferation curve indicated faster proliferation of co-cultured HMSC-bm than HMSC-bm. Karyotyping analysis showed that the chromosome number of co-cultured HMSC-bm was hypotriploid and triploid and the chromosome was grossly abnormal. The expression of HDAC4 protein in co-cultured HMSC-bm significantly increased compared to that in HMSC-bm (p<0.01). Conclusion Pulmonary adenocarcinoma microenvironment could influence the proliferation speed and the genetic stability of HMSC-bm .

Key words: HMSC-bm, pulmonary adenocarcinoma microenvironment, genetic stability, chromosome