Abstract: Objective To test db/db mouse kidney tissue of the cysteine-rich acidic secreted proteins(SPARC)expression. Methods RT-PCR, Western blot and immunofluorescence method detection db/db mouse kidney tissue of SPARC mRNA and protein expression. Results SPARC highly expressed in db/db mouse kidney tissue. Conclusion SPARC in the db/db mouse kidney tissue showed high expression may be related to the occurrence and development.