Abstract: Objects Investigate the changes of inflammatory factors secreted by macrophages exposed to oxidative stress, and those effect on the proliferation of vascular endothelial cells and smooth muscle cells. Methods The groups described as followings: control, macrophage, 0.5mmol/L H2O2, macrophage+0.5mmol/L H2O2. The growth curves of VECs and VSMCs under different conditions were measured by CCK-8. The IL-6, IL-10, MCP-1, TNF-α and VEGF were detected by ELISA. The level of PCNA, cyclinD1 and eNOS were analysised by Western blot. Results The proliferation of control group was higher than the others in VEC(P<0.05), while, in VSMC, the control group was lower than the macrophage group(P<0.05). And, in both VEC and VSMC, macrophages+0.5mmol/L H2O2 conditioned medium groups were higher than 0.5mmol/L H2O2 groups(P<0.05), while were lower than macrophage groups(P<0.05). The secretions of IL-6, IL-10, and MCP-1 in macrophages were increasing with time and H2O2 concentration(P<0.05), while the amount of TNF-α didn’t change significantly. Only the concentration of H2O2 reached 1.0mmol/L, did the secretion of VEGF increase significantly(P<0.05). Conclusions The oxidative stress treatment can affect the secretions of inflammatory cytokines secreted by macrophages, and then affect the proliferations of vascular endothelial cells and smooth muscle cells.

Key words: Oxidative stress, Inflammatory factors, Proliferation