Abstract: Objective To construct full length infectious cDNA clone of enterovirus 71. Methods A cDNA fragment with a T7 promoter at the 5’ end and a poly (A) tail at the 3’ end was amplified by RT-PCR from the genome of the EV71/87-2008 Xi’an strain directly. This fragment was then ligated to pBR322 via SalI and BspEI site. Then the in vitro synthesized RNA transcripts were transfected into RD cells to produce the rescued virus. The negative-strand viral RNA was detected from the RD cells lysates by RT-PCR. The one step growth curve of recovered EV71 was measured by plaque assay. Results The full length cDNA clone of EV71/87-2008 Xi’an was constructed and the infectious of this clone was validated successfully. The EV71 recovered virus was proved functionally identical to its template. Conclusion Results suggest that the infectious clone of EV71/87-2008 Xi’an strain was constructed successfully.

Key words: Key words: EV71, infectious clone, one step growth curve