Abstract: To investigate the impact of S100A6 on β-catenin and its molecular mechanism in human breast cancer cell line MCF-7. Methods MCF-7 cells were infected by recombinant adenoviruses carrying human S100A6 and its siRNA gene, AdS100A6 and AdsiS100A6 respectively. RT-PCR,Western blot and immunocytochemistry were used to detect expression and/or distribution of β-catenin,E-cadherin and p-GSK3β. Results In MCF-7 cells, S100A6 increased the protein level of β-catenin（P<0.01）,but no significant difference in transcriptional level of β-catenin between S100A6 group and control group（GFP group）（P>0.05）; The mRNA,protein level and distribution of E-cadherin had no significant difference between S100A6 group and control group（P>0.05）; S100A6 increased phosphorylation of GSK3β（P<0.01）.Conclusion S100A6 may upregulate β-catenin by promoting phosphorylation of GSK3β，rather than via effect on E-cadherin.

Key words: S100A6, β-catenin, E-cadherin, GSK3β