Abstract: Objective To screen the protein which can interact with Dok6 and explore the potential function . Methods Immunoprecipitation, western blot, and sliver staining were employed to screen the candidate proteins interact with Dok6. liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the binding proteins of Dok6. Finally, we analyzed its potential physiological functions by bioinformatics prediction. Results After screening the binding proteins of Dok6 with IP and LC-MS/MS, 26 candidate binding proteins were identified. These proteins are involved in many physiological processes in the body. In order to narrow the scope of the study, we selected nine proteins associated with nervous system development and intracellular signal transduction as object of study for following study of the function. Conclusions The potential Dok6 downstream proteins identified by mass spectrometry and bioinformatics will provide some clues to determine physiological functions and related molecular mechanisms of Dok6 .

Key words: Dok6, Immunoprecipitation, mass spectrometry identification, functional analysis