Abstract: Objective: To construct a tumor specific expressing GFP plasmid modulated by hTERT promoter. Method: hTERT promoter was cloned by PCR, and used to substitute the CMV promoter of pEGFP-C3 plasmid by means of genetic engineering to form recombinant plasmid(hTERTp-EGFP), which was subsequently used to transfect tumor cell and non-tumor cell respectively. Results: The recombinant plasmid hTERTp-EGFP was successfully established, and its post-transfection expression was observed in tumor cells but not in non-tumor cells. Conclusion: Recombinant plasmid hTERTp-EGFP has the potential of tumor specific expression.

Key words: telomerase, hTERT－promoter, tumor, plasmid