Abstract: Objective To study effect and the mechanism of As2O3combined with recombinant canstatin protein on tumor vascular endothelial cell proliferation, migration, angiogenesis and apoptosis.Methods The cells were induced to differentiate into tumor-derived endothelial cells ( Td-ECs) by co-culturing with supernatants of HepG2 cells. The anti-effect of As2O3 and canstatin on Td-ECs was detected with cell migration, flow cytometry, blood vessel formation assay. Results As2O3 ,canstatin and the combined all effected on Td-EC in vitro inhibited their migration cells respectively: 32.60±1.51、26.60±1.14、21.00±1.87，significantly higher than control group 50.60±2.30（p＜0.01）,and promoted apoptosis, blood vessel formation.The effect of combined was more significant.Conclusion As2O3 combined with recombinant canstatin protein in vitro inhibits Td-EC proliferation, migration, angiogenesis and promotes apoptosis.

Key words: As2O3, Canstatin tumor-derived endothelial cells, Apoptosis, Migration, Angiogenesis