Abstract: Objective: To establish a simple and less toxic method for protein staining following SDS-PAGE. Methods: After electrophoresis, the gel was fixed temporarily, and then stained with the Coomassie Brilliant Blue G-250 (CBBG-250) solution containing aminosulfonic acid. De-staining was not required. Results: The sensitivity of this method was higher than that of the classical CBBR-250 staining method. Moreover, toxic solvents such as methanol, though commonly used in the staining and de-staining solutions, were eliminated. Conclusion: The improved method has a series of advantages including high sensitivity, clear background, dye-saving, direct color development without de-staining, and having a safe and less toxic recipe.

Key words: [Key words] electrophoresis, polyacarylamide, protein staining, Coomassie Brilliant Blue